A genetic, genomic, and computational resource for exploring neural circuit function.

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eLife. 2020 Jan 15;9. doi: 10.7554/eLife.50901

A genetic, genomic, and computational resource for exploring neural circuit function. Reiser LabRubin LabFlyLightInvertebrate Shared Resource

Davis FP, Nern A, Picard S, Reiser MB, Rubin GM, Eddy SR, Henry GL

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Abstract

The anatomy of many neural circuits is being characterized with increasing resolution, but their molecular properties remain mostly unknown. Here, we characterize gene expression patterns in distinct neural cell types of the visual system using genetic lines to access individual cell types, the TAPIN-seq method to measure their transcriptomes, and a probabilistic method to interpret these measurements. We used these tools to build a resource of high-resolution transcriptomes for 100 driver lines covering 67 cell types, available at http://www.opticlobe.com. Combining these transcriptomes with recently reported connectomes helps characterize how information is transmitted and processed across a range of scales, from individual synapses to circuit pathways. We describe examples that include identifying neurotransmitters, including cases of apparent co-release, generating functional hypotheses based on receptor expression, as well as identifying strong commonalities between different cell types.

PMID: 31939737 [PubMed - indexed for MEDLINE]

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A genetic, genomic, and computational resource for exploring neural circuit function.

Abstract

PMID: 31939737 [PubMed - indexed for MEDLINE]

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